Category Archives: NorBOL

What’s growing on the shell? – Insights into the Diversity of Hydractiniidae in Norway

This summer I started my master degree project at the University Museum of Bergen and joined the research of Luis Martell and Aino Hosia. I’m a student in the program ‘Biodiversity & Systematics’ at the University of Stockholm and for my 1-year thesis project I wanted to learn more about hydrozoans. Looking for hydrozoan biologists, which there are not so many, I came across the project NorHydro led by Luis and Aino and I decided to go to Bergen to study and learn more about this fascinating group.

My thesis project especially focuses on the diversity of the hydrozoan family Hydractiniidae. Most commonly hydractiniids are encountered on snail- or hermit crab shells where they can build a mat of polyps. Those shells are often inhabited by other animals and the polyps feed on the left-over meals of those and in return defend their host against predators. With their small tentacles, which are equipped with hundreds of cells containing venomous stingers (the nematocysts) they are great predators and catch tiny animals from the plankton.

Schuchertinia allmanii growing on Pagurus pubescens hermit crab. Picture credit: Bernard Picton

Each individual polyp is connected to each other and build a colony of polyps with different functions. Some act as food suppliers, some as reproductive polyps. From those polyps some hydractiniid species grow tiny medusae (jellyfish) which will be released in the water column. The medusa is morphologically comparable to the polyps, equipped with tentacles, a stomach, gonads and nematocysts – but living upside down and not attached to the sea floor. With their ability to swim around they contribute to disperse themselves in the water and can produce a lot of offspring.

Video of a Pagurus bernhardus hermit crab. A hydractiniid polyp colony is growing on the underside of the shell. Video credit: Lara Beckmann

Several species in this family are often used in development biology or immunology research. For example the species Hydractinia echinata led among other organisms to the discovery of stem cells and is still widely used as a so-called model-organism nowadays. This is because this animal group shows a high ability to regenerate and if you cut a hydroid polyp in two pieces, it will just re-grow again to its initial state.

The life-cycle in hydractiniids can include a polyp and medusa stage. Species of the genus Podocoryna release medusae from their colony. Other genera in this family develop only reduced medusae and lack a free-swimming jelly. Illustration credit: Lara Beckmann

The hydromedusa Podocoryna borealis is released from a polyp colony that can grow on various substrates such as snails or worm tubes. Picture credit: Lara Beckmann.

But despite this spotlight to some species, the diversity of this family is still poorly known and there is little attention to hydractiniid species because of their inconspicuousness and difficult identification. To highlight the problem I did some research: how many and what species are commonly recorded in Norway? Which species could potentially occur in this region according to species descriptions? In private observations and ecological surveys only three species were commonly recorded. However, our preliminary results suggest at least 6 species in Norwegian waters with some surprisingly frequent species that are rarely recorded in surveys. This indicates, that there happens a lot of misidentification in the field and we need to get a better estimate of the true diversity of Hydractiniidae in this region in order to improve awareness and to prevent misidentification in the future.

Two polyps of a colony of the species Clava multicornis. You can see reproductive units (so-called sporosacs) growing on the polyp body, which will release eggs or sperm when fully grown. Picture credit: Luis Martell

In order to do that I study this group in much detail. A great advantage of being a taxonomists in these days is that we can use a variety of different sources of information to develop and test for species hypothesis. This so-called integrative approach includes morphological, ecological and genetical information in order to define taxa diversity from several perspectives.

The first step in this process is to gather polyps and medusae of Hydractiniidae from various spots in Norway ranging from Olso, via Bergen and up north to Bodø. I will use already collected samples from previous years and hopefully will also be able to add some more samples from field-trips in the next months.

Getting the plankton-net back on board – and Maryam and me trying to open the jar carefully without spilling the fragile plankton inside. After removing the jar from the net, we directly pick the hydrozoans and put them in a cold environment, so that we can look at them alive when we are back in the lab. Picture credit: Maryam Rezapoor

With fresh material the first step is to take pictures of the living animal. Since hydrozoans tend to shrink and lose a lot of it characteristic traits in the ethanol preservative it is important to take pictures right away when the animal is still alive. Furthermore the samples are identified using a microscope, identification keys and additional literature. With additional collected data such as habitat, location, sample depth and so forth we create a large database for our samples and develop so-called e-vouchers: an electronic data storage for each individual to ensure to gather and store as much information as possible. That makes it possible to go back to the database and look at the specimen data e.g. where did it live? On what substrate was it growing on? How deep was the sampling site? This way I don’t only rely on the preserved material but can easily use the e-voucher material and the additional collected data that can be useful for further analyses.

Besides the morphological and ecological data I also include molecular information: what does the DNA tell us about the specimen? For that I use three different genetic markers (short DNA fragments, or genes) to see how those differ in their sequence of bases between the sampled hydractiniid specimen. In the course of evolution, DNA sequences change due to selection, genetic drift, gene flow and random mutations. That is why we are able to infer evolutionary relationships between biological entities – in this case we are interested in species – and with mathematical models and some simplified assumptions we can delimit species based on their molecular attributes.

Me in the DNA lab waiting for the first results of lab work for this project! Picture credit: Maryam Rezapoor

In order to do so, I will process the sampled material in the lab. First, I extract the DNA from a tiny piece of polyp or medusa tissue and then amplify the specific target region using PCR (Polymerase-Chain-Reaction). This short DNA fragment will further be sequenced which outcome I will check, process and analyze using different computer programs and algorithms. The final results will be summarized and visible in so-called gene trees, revealing the evolutionary history of those three different DNA markers. I will use the gene genealogies to delimit the species and observe how much diversity of hydractiniids we have in Norway.

Using specific genes also facilitate us with species barcodes which are used to create a reference library. This in return enables researchers to identify a specimen without the need to identify the sample using its morphology. This can be the case e.g. for metabarcoding projects (sequencing a mass of DNA from unknown origin) or if samples are unidentifiable. The scientist will then sequence the barcode gene and compare it to the reference library and will eventually be able to tell the species. Those barcodes can be informative in biodiversity studies but by no means substitute taxonomists, which are still needed to describe and identify species when no DNA is available.

A monograph of the Gymnoblastic or Tubularian hydroids by George James Allman, published in 1870. I found this book in the library of the Tjärnö laboratories in Sweden. Allman was a pioneer in hydrozoan research and described many species. Nowadays it is much easier to access original descriptions since many book are available online. You can find a PDF* of this book in the Biodiversity Library. Picture credits: Lara Beckmann

Also, the DNA does not help us when we have no reference we can map the specimen to in order to determine the species. That is why this master project also aims to create a barcode-library for the species in the family Hydractiniidae.

What I really like about this project is that it contributes to our basic understanding of the diversity in our oceans – in this case even in two completely different environments: the water column as well as the seafloor.

Even if hydractiniids are just a little part of the ecosystem, they play their role and influence the ocean in their own way. Also, my work is super diverse and I need to be an allrounder in many respects: trying to find species descriptions I search and read taxonomic literature from the 19th century (coming in Latin, German, English, Swedish or French). Then I work in the laboratory where I combine traditional working methods like drawing, microscopy or photography with modern techniques from DNA sequencing to computational science and bioinformatics.

I love this kind of work, and it is great to contribute just a little bit to reveal the mysteries of our oceans.


– Lara

You want to learn more about hydrozoans and why it is important to study them? Read more about it in my blog article for Ecology for the Masses (link).

Also, keep up with the activities of NorHydro (link to project home page) here in the blog, on the project’s facebook page (link) and in Twitter with the hashtag #NorHydro.

*Link to pdf of A monograph of the Gymnoblastic or Tubularian hydroids by George James Allman

Brattström baby, HYPCOP goes offshore!

Last days of November HYPCOP spend two days (26th & 27th) offshore. We had the possibility to join some sampling efforts of NorHydro and others on the research vessel Hans Brattström.

Research Vessel Hans Brattström ready early in the morning, photo Cessa Rauch

This vessel is owned by the University of Bergen and operated by the institute of Marine Research (IMR, Havforskningsinstituttet).

H. Brattström is used 200 – 230 days a year along the West coast of Norway. It has the capability of operating different sampling gear, which makes it useful for multiple projects, studying a variety of marine organisms, from fish, to worms, jellyfish, and yes, also copepods!

On the first day HYPCOP joined NorHydro consisting of Luis Martell (UiB) and Joan Soto Angel (Sars):

NorHydro team and HYPCOP; from ltr Cessa Rauch, Luis Martell and Joan Soto Angel, photo Cessa Rauch

Plankton net being lowered in the ocean with some early morning sun, photo Cessa Rauch

 

 

The main sampling gear consisted of a large plankton net that was slowly dropped to 660m, 245m and 128m depth.  We sampled close to Bergen in Raunefjord, Krossfjord and Fanafjord.

Sampling for jellyfish needs to be done with caution, with the net going up to fast, the animals will just fall apart because of the pressure. So, a depth of 660m can take up to an hour and more before we could see the results.

 

 

Joan Angel Soto scanning the shore for birds, photo Cessa Rauch

During the waiting times we didn’t let our time go to waist, with binoculars we scanned the air and shore for birds.

After waiting for some time, the plankton net was brought back on board and contained, besides jellyfish and other pelagic planktonic dwellers, many million copepods. Mostly consisting of a few species. One of the species had a distinguishable blue egg sack, this is Paraeuchaeta norvegica (Boeck, 1872). This species is an active predator that feeds on other (smaller) copepods by rapidly jumping on them and catching their prey with their large maxillipeds (mouthparts).

 

 

The second day HYPCOP joined head engineer Bjørn Reidar Olsson (UiB) and PhD student Miguel Meca (UiB)

HYPCOP (Cessa Rauch left) joining Miguel Meca (middle) and Bjørn Olsson (right), photo Cessa Rauch

They were looking for shark teeth and polychaetes (marine worms) respectively and used the grab, which is perfect for benthic copepod sampling. The grab is basically a big metal clamshell that collects sediment from the seafloor. Working with grab samples gets dirty very quickly, you have to wash through the sediment to find your animals.

The grab with Cessa Rauch (HYPCOP left), Miguel Meca (middle) plus operator Bjørn Frode Grønevik (right), photo Bjørn R. Olsson

Most of the sediment was filtered out in order to find our copepod friends. Although less plentiful in comparison to the plankton net sampling the previous day, we still found some copepods hiding in the dirt. At moment of this writing, the the copepod species we collected have not be named yet, however, the last months we have been experimenting with barcoding the first batch of 60 different specimens. We had a 43% success rate. Usually, marine invertebrates have a success rate between 40 – 70%, so it was still within the margin, but not with a lot of enthusiasm. HYPCOP will spend the remainder of 2020 and the beginning of 2021 in the laboratory figuring out what the culprit of this low success rate could be.

For HYPCOP this will be the last blog before the Christmas holidays and the New Year. Therefore, we want to take the opportunity to wish you happy holidays and hope to see you around in 2021 with from us more copepod news to share!

-Cessa

Follow HYPCOP @planetcopepod

Instagram, for pretty copepod pictures https://www.instagram.com/planetcopepod/

Twitter, for copepod science news https://twitter.com/planetcopepod

Facebook, for copepod discussions https://www.facebook.com/groups/planetcopepod

See you there!

Fieldwork at Sletvik Fieldstation!

From Monday 12th of October till Monday the 19th a bunch of different projects funded by the Norwegian taxonomy initiative travelled up North together to meet up with researchers from NTNU in the NTNU Sletvik field station.

Front of Sletvik fieldstation main building, photo credits Nina T. Mikkelsen

Sletvik fieldstation is NTNU owned and is a short drive from Trondheim. The Germans built the station during the Second World War. Ever since it has been used as a town hall, a school and a shop. In 1976 the NTNU University took over the building and transformed it into a field station, which it remains ever since. The entire station contains of two buildings that has room for a total of 75 people (Before Corona). The main building has a kitchen, dining and living room plus a large teaching laboratory, a multilab and two seawater laboratories. Besides it has bedrooms, sauna, laundry rooms, and showers, fully equipped! The barracks have additional bedrooms and showers, all in all, plenty of space.

 

From the Natural History Museum of Bergen, 5 current running projects would use the NTNU fieldstation facilities for a week in order to work on both fixed as well as fresh material. Besides HYPCOP (follow @planetcopepod), we had Hardbunnsfauna (Norwegian rocky shore invertebrates @hardbunnsfauna), Norhydro (Norwegian Hydrozoa), Norchitons (Norwegian chitons @norchitons) and NorAmph2 (Norwegian amphipods) joining the fieldwork up North!

Lot of material needed to be sorted, photo credit @hardbunnsfauna / Katrine Kongshavn

 

At the Sletvik fieldstation, a lot of material from previous fieldwork was waiting for us to be sorted.

For HYPCOP we wanted to focus mostly on fresh material, as this was a new location for the project. And not just new, it was also interesting as we have never been able to sample this far north before.  Almost every day we tried to sample fresh material from different locations around the fieldstation

Cessa and Francisca on the hunt for copepods, photo credits Katrine Kongshavn)

On top of that we aimed to sample from different habitats. From very shallow heavy current tidal flows, rocky shores, steep walls, almost closed marine lakes (pollen called in Norwegian) and last but not least, sea grass meadows

Different habitats give different flora and invertebrate fauna, photo credits Nina T. Mikkelsen

Sampling we did by either dragging a small plankton net trough the benthic fauna or the most efficient way, going snorkeling with a net bag

Ready for some snorkeling with Cessa and August, photo credits Torkild Bakken

Benthic copepod species tend to cling on algae and other debris from the bottom, so it is a matter of collecting and see in the laboratory whether we caught some copepods, which, hardly ever fails, because copepods are everywhere!

Copepods are difficult to identify due to their small nature, differences between males, females and juveniles’ and the high abundance of different species. Therefore, we rely heavily on genetic barcoding in order to speed up the process of species identification. So, after collecting fresh material, we would make pictures of live specimens to document their unique colors, and then proceed to fixate them for DNA analyses.

Yet unidentified copepod species with beautiful red color, photo credits Cessa Rauch

Winter Wonderland! Photo credits Cessa Rauch

The other projects had a similar workflow so you can imagine, with the little time we got, the Sletvik fieldstation turned into a busy beehive! One week later we already had to say goodbye to the amazing fieldstation, and after a long travel back (even with some snow in the mountains), we finally arrived back in Bergen where unmistakably our work of sorting, documentation and barcoding samples continued!

If you are interested to follow the projects activity, we have social media presence on Twitter (@planetcopepod, @hardbunnsfauna, @norchitons), Instagram (@planetcopepod, @hardbunnsfauna, @norchitons) and Facebook (/planetcopepod /HydrozoanScience).

 

-Cessa

HYPCOP workshop at the IMR fieldstation in Flødevigen

HYPCOP (Hyperbenthic Copepoda) is a young project starting date May 2020 with joined efforts between researchers from the Institute of Marine Research (IMR; Tone Falkenhaug), Natural history museum of Bergen (UiB; Cessa Rauch, Francisca Correia de Carvalho, Jon Anders Kongsrud) and Norwegian Institute for water research (NIVA; Anders Høbæk). If you want to read more about what HYPCOP entails, read it all in our previous blog here: link to HYPCOP kickoff blog.

We were already off with a good start with having quite some fieldwork and sampling done this Summer in and around Bergen, Killstraumen, Lillesand, Drøbak and now with our most recent trip to Flødevigen.  During week 35 (24 – 28 August), all the different researchers from HYPCOP traveled to the IMR fieldstation in Flødevigen to participate in a sampling excursion. It was a special event because it was the very first time since the project started that all the collaborators would meet, in real life! We had many meetings via the digital platforms but working together face to face is quite a different and more pleasant experience (Picture 1).

Team members at the field station; from ltr: Anders Hobæk (NIVA Bergen, Jon Kongsrud (UoB, Tone Falkenhaug (IMR), Cessa Rauch and Francisca de Carvahlo (UoB)

The HYPCOP project is special in many ways; besides the involvement of many different institutes, the team deals with quite a steep learning curve. As off now there are very few hyperbenthic copepoda taxonomists in the world and none in Norway. Anders Hobæk has experience with freshwater copepoda, however his skills are transferrable to the marine species, which helps us a lot. Tone Falkenhaug has experience with copepods from previous projects (COPCLAD; Inventory of marine Copepoda and Cladocera (Crustacea) in Norway). However, the difference between COPCLAD and HYPCOP is the habitat: COPCLAD invented the pelagic realm, while HYPCOP focuses on the Hyperbenthos.

The copepod light trap from Tone Falkenhaug

We decided to use the few days we had together to start from scratch, which meant, first getting some samples from the water.

We all used different techniques to make sure we got copepods from different habitats;

Jon went for snorkeling;

Anders brought his miniature plankton net,

and Tone set her light traps out.

 

This ensured that we had a higher chance of getting different species to look at. Next we would look at our freshly caught samples under the microscope and tried to sort them based on morphotypes (as much as that is possible, as they move fast!).

Copepods can actually have very nice colors! Therefore, we prefer to take live images of the animals as well as when they are fixed on absolute ethanol. So, after sorting them, we continued to make pictures before fixing the animals ready for the next steps.

A colourful specimen, as of yet unidentified

After fixing we experimented with different staining methods in order to make the exoskeleton of the copepods more visible for detecting important morphological features. An important part for species identification is studying the individual body parts of the animals, like the antennae, the individual pair of legs, the claws (maxillipeds).

The animals also have differences between males and females, so it is key to make sure that you identify it as the same species! With morphological identification it is important to also keep some specimens aside for genetic studies. Only when the DNA barcode and the morphological identification agrees we can be certain about the right species identification! As you can read there’s a lengthy process involved before we have the right identification of a copepod specimen and there are hundreds of species described for Norway alone! It is truly very extensive research! Follow us on Twitter and Instagram @planetcopepod to follow our story, or become a member of our planetcopepod Facebook group for the latest news and finding!

See you there!

-Cessa

Sea slugs of Southern Norway; farewell but not goodbye!

A note from the Norwegian Taxonomy Initiative project (artsprosjekt) “Sea Slugs of Southern Norway” (project home page), which ran from 2018 to the end of April 2020.

Dear all,

The Sea slugs of Southern Norway project reached its terminus at the end of April, with sending the last reports of our collection and research efforts to Artsdatabanken (the Norwegian Biodiversity Information Centre).

What we have been able to build up these last two years is of immense importance for the scientific collections of the Natural History Museum of Bergen (University of Bergen)  and for (Norwegian) biodiversity research.

Sea slugs of Southern Norway managed to collect over 1000 lots covering 93 different sea slug species, of which 19 are new for Norway and a few new to science (we are working on it!).

Below are photos of the species that were collected at different sampling events.  The photos are made either by the researchers associated with the project, or by the amazing team of citizen scientists.

Look at these beauties!

This would absolutely not have been possible without the special effort of our knowledgeable citizen scientists, and we would like to use this opportunity to name a few that were extraordinarily productive during the last years and provided the project and the Museum with valuable samples; Nils Aukan, Roy Dahl, Viktor Grøtan, Heine Jensen, Tine Kinn Kvamme, Runa Lutnæs, Ole Christian Meldahl, Jenny Neuhaus, Bjørnar Nygård, Anders Schouw, Erling Svensen, Cecilie Sørensen, Mona Susanne Tetlie, Anne Mari with Ottesen, Mandal Dykkerklub, Hemne Dykkerklubb, Slettaa Dykkerklubb, SUB-Studentes Undervannsklubb Bergen, Larvik Dykkerklubb, Sandefjord Dykkerklubb, and all the others that made big and small contributions.

A big thank-you to all contributors!

Would you like to know more about the citizen scientists part of the project? Check out this paper (starts on page 23) by Cessa and Manuel: Sea Slugs of Southern Norway: an example of citizens contributing to science.

Mandal team

One of the core components of the projects success was our outreach effort on all kind of social media platforms. During these two years these platforms got much more traffic than we initially thought; apparently we have many Norwegian sea slug fans, within and outside of Norway!

Therefore we decided to continue with our outreach efforts to keep everyone engaged and up to date about these wonderful animals in our ocean backyard, but with some minor adjustments. Some of you might have already noticed a few changes during the last days on the Facebook page  and our Instagram account. From today onward, the social media pages will cover sea slugs of all of Norway, and is now named accordingly. We also welcome a new admin to the facebook group: Torkild Bakken of NTNU University Museum. Welcome Torkild, the more expertise the better, so we are very happy to have you onboard!

We encourage everyone in this community to continue to be active and share your findings and knowledge with other.

Let’s carry on enjoying the wonderful world of sea slugs of Norway!

 

-Cessa & Manuel

 

HYPCOP kickoff!

Follow us at @planetcopepod!

Tuesday may 19 was the first fieldwork of the new project called Hyperbenthic Copepoda (HYPCOP). You can read more about the field work and see some photos and videos from the field in the previous blog post. 

Copepoda are small crustaceans that are found all over the world in both marine and freshwater habitats. Species can be planktonic (drifting in the sea water) or can be parasitic and a large diverse group of them live on algae in the hyperbenthic (living near the bottom) zone. Copepoda are very important food source for many organisms like small fish, they are on the bottom of the food pyramid, together with other zooplankton. Without copepods, a lot of bigger animals would no exists. Despite being so important, not much is known about the biodiversity and taxonomy of these animals, especially that of the species that live near the bottom.Some species like Calanus finmarchicus are the main nutritional basis for many fish species, and therefore of great importance for the Norwegian fish strains.  Therefore Artsdatabanken is funding the new project HYPCOP in order to unravel the biodiversity and taxonomy of hyperbenthos copepods. With special focus on the species in the group Harpacticoida that live in the water masses just above or near the bottom. Copepods from shallow water will be collected in coastal areas, in deep fjords and on the continental shelf.

The Institute of Marine Research (IMR), Natural history museum of Bergen (UiB), Norwegian Institute for water research (NIVA) and the Norwegian Barcode of Life (NorBoL) are working together to survey the diversity of marine copepods in Norwegian waters and expect to find and describe species that are new to science and new for Norway! Currently some of the taxonomic competence in Norway is lacking, but through collaboration with foreign experts this knowledge will increase among Norwegian researchers and students!

The projects duration will run from 2020 until 2023 and last week was the official kickoff with some fieldwork to get fresh material to work with! Together with the project Hardbunnsfauna we drove to a local favorite collection site of us; Biskopshavn; very close to Bergen city center. Around the hard substrate we found lots of freshly grown algae that contained many small animals for us to collect! In order to get good quality samples we needed to be in the water and snorkel. With plankton nets we collected algae and sieved the water column catching the smallest of the animals; copepods!

And even though this was just a first test of equipment and collection methods, it was not without success. Back in the lab the microscope revealed the beautiful and diverse world one drop of seawater contains. A lot off small crustaceans and of course the copepods were omnipresent.

Our findings had to be shared and especially for #InternationalDayForBiologicalDiversity the copepods cannot be left out as they from such an important group! See for yourself the beauty of our copepod planet!

-Cessa

Field work in Biskopshavn

Happy International Day for Biological Diversity 2020!
On this day, we wanted to share a few glimpses of our most recent field work:

We were finally able to – with some precautions in place – resume our field activities again this Tuesday; we had a lovely day trip in the sun to Biskopshavn, a locality just a few minutes drive away from the lab.

Here we collected animals from the shallow sub-littoral (from just below the tide mark to ~3 m depth) for the new project on Copepoda (see more about that here), and for Invertebrate fauna of marine rocky shallow-water habitats (Hardbunnsfauna).

Below is a short video from the field & lab (including the inevitable Littorina on the lam!), and a few of our findings from the day.

This is a polychaete in the family Syllidae. If you look at the tail end on the top image, you can see that it is about to breed: these animals can do so with schizogamy, which is the production of stolons (enlarged in lower image) which are budded off and become pelagic, swimming away to breed. The stolons form complete new animals, but differ from the stock animal in a number of respects, such enlargement of the eyes, reduction of the gut, and different musculature. The stolons die after breeding.

One of the animal groups Hardbunnsfauna is focusing on is the Bryozoa, or moss animals. Pictured is a Bowebankia spp. Due to COVID we haven’t been able to host our international specialists here this spring. We are amassing a nice collection of animals, and do our best to identify them – we will  begin preparing plates for DNA barcoding soon, and then involve the taxonomists once we have the results.

-Katrine, Cessa & Jon

Meet ZMBN 130407!

How much information do you think we have on the animals in our collections? 🤔

Quite a lot more than you might think, and here to help us show you, we have a small snail from the shore. Meet specimen #ZMBN 130407, a Littorina saxatilis 🐌 (rough periwinkle/spiss strandsnegl).

We collected it one year ago on our fieldwork up North, in Tendringsvika near Tjeldsund (Troms): our northernmost station on the trip.

Tendringsvika in Troms

Here’s a short video of the habitat: notice how the sea urchins dominate once we get below the intertidal zone!

To be able to use the Invertebrate Collections for research, we need to know quite a lot about each animal (“specimen”). Standard information would be where, when and how it was collected, who collected it, who identified it (and revisions), notes about the habitat, images if any, and the museum number that it is registered within our database.

A screenshot of how it may look when a specimen is registered in our database

If there is genetic data – like here, a DNA barcode as part of NorBOL – we also need the genetic information. This information is stored in the international barcode library BOLD (BOLDSystems.org), where it is organised in projects containing information linked to the physical specimen, and to the DNA.

Small snail, much data!

If you look at the lower right corner, you will find information about specimens that have identical DNA sequences, and who are therefore grouped together in what is called a BIN in BOLD (/OTU). Most of the other specimens with identical DNA barcode have also been identified to Littorina saxatilis, but not all…that’s one reason to keep the animals in museum collections, so that identifications can be re-checked if needed 🔬.

Through our project (hardbunnsfauna) on shallow water hard bottom fauna from Norway, we are helping build a good DNA barcode library of species that occur in Norway – with reference (“voucher”) specimens in the scientific collections of the University Museum of Bergen, and with our partner, NTNU University Museum.

-Katrine

Science Communication – Creating Scientific Illustrations

What on earth is this going to become?

I (Katrine) recently attended a course on how we can use illustrations to (better) communicate our science.

The course was offered as a joint effort of four Norwegian research schools: CHESS, DEEP, ForBio and IBA, and I got my spot through ForBio (Research School in Biosystematics).

The course was taught by Pina Kingman, and covered a lot of different topics in four days, from messy drawing with charcoal to using graphic software for digital illustrations:

  • Principles of design and visual communication
  • How to apply these principles to illustration and graphic design, which in turn will inform all visual material you might want to create, including; graphical abstracts, presentation slides, poster presentations, journal articles, graphs, data visualisation, project logos, animations and outreach material.
  • Best practices for poster and slide presentation design
  • Step by step method on how to draw your own research
  • Introduction to sketching by hand
  • Crash course in digital illustration with mandatory pre-course digital tutorials

Now, we were sternly told on day 1 that we were not allowed to say that we could not draw…but let’s say that some people have more of an affinity for it than I do – see above for proof! None the less, a concept was to be developed, discussed and improved during group work, and ultimately transformed into a digital illustration by the end of day 4.

Most of my fellow students were creating something related to their ongoing research, such as an illustration to be used in a paper of their PhD. On the last day we presented our work for the class, and got the final feedback from the group. Spending a whole day looking at cool graphics and learning about people’s work on such varied topics as water flow in magma, colour patterns on Arctic rays, better diagnosis of tuberculosis, and ecosystem modelling was really enjoyable, and the feedback I got was very helpful.

I opted for an outreach-approach, creating a lot of small illustrations that will be individually useful in future presentations and such, and which could be combined into a small comic about our scientific collections. The comic has been shared on Twitter and Instagram (do follow @hardbunnsfauna!), and now here:

The end product of the course; a short introduction to our scientific collections, how we work, and how we integrate data such as DNA-barcodes and morphological traits of the animals to do our research!

Thank you to Pina, Mandy (& the other arrangers), and the class for a wonderful learning environment and a fun couple of days!

-Katrine

Field season’s end

Sletvik field station, October 15th-23rd 2019

We wanted to make a write-up of the last combined fieldwork/workshop we had in 2019, which was a trip to the marine field station of NTNU, Sletvik in Trøndelag, in late October. From Bergen, Luis (NorHydro), Jon, Tom, and Katrine (Hardbunnsfauna) stuffed a car full of material, microscopes, and drove the ~12 hours up to the field station that we last visited in 2016.Beautiful fall in Trøndelag

There we joined up with Torkild, Aina, Karstein, and Tuva from NTNU university museum, students August and Marte, and Eivind from NIVA. We also had some visitors; Hauk and Stine from Artsdatabanken came by to visit (if you read Norwegian, there’s a feature about it here), and Per Gätzschmann from NTNU UM dropped by for a day to photograph people in the field.

Most of the workshop participants lined up Photo: Hauk Liebe, Artsdatabanken

During a productive week the plan was to work through as much as possible of the material that we and our collaborators had collected from Kristiansand in the South to Svalbard in the North. Some of us went out every day to collect fresh material in the field close to the station.The Artsprosjekts #Sneglebuss, Hardbunnsfauna, NorHydro, and PolyPort gathered at Sletvik, and with that also the University museums of Trondheim and Bergen. Of course we were also collecting for the other projects, and the museum collections.

One of the things Hardbunnsfauna wanted to do whilst in Sletvik was to pick out interesting specimens to submit for DNA barcoding. This means that the animals need to be sorted from the sediment, the specimens identified, and the ones destined to become barcode vouchers must be photographed and tissue sampled, and the data uploaded to the BOLD database. We managed to complete three plates of gastropods, select specimens for one with bivalves, and begin on a plate of echinoderms, as well as sort through and select quite a few crustaceans and ascidians for further study.

Collecting some fresh material was particularly important for NorHydro because the hydroids from the coasts of Trøndelag have not been thoroughly studied in recent years, and therefore we expected some interesting findings in the six sites we managed to sample. We selected over 40 hydrozoan specimens for DNA barcoding, including some common and widespread hydroids (e.g. Dynamena pumila), some locally abundant species (e.g. Sarsia lovenii) and exceptionally rare taxa, such as the northernmost record ever for a crawling medusa (Eleutheria dichotoma). We also used a small plankton net to catch some of the local hydromedusae, and found many baby jellyfish belonging to genus Clytia swimming around the field station.

Plan B when the animals (in this case Leuckartiara octona) won’t cooperate and be documented with the fancy camera; bring out the cell phones!

It was a busy week, but combining several projects, bringing together material spanning all of Norway, and working together like this made it extremely productive!

Thank you  very much to all the participants, and to all the people who have helped us gather material so far!

-Katrine & Luis