Tag Archives: bryozoa

Door #15: The eye of the beholder

It’s funny to see the different reactions to fresh material that comes in to the museum;  the exhibition team had  received some kelp that will be pressed and dried for the new exhibitions (opening fall 2019), and I ducked in to secure some of the fauna sitting on the kelp before it was scraped off and discarded. For the botanists, the animals were merely a distraction that needed to be removed so that they could deal with the kelp, whilst I was trying to avoid too much algae in the sample as it messes up the fixation of the animals.

I chose the right shirt for the day- it’s full of nudibranchs! (photo: L. Martell)

 

I then spirited my loot into the lab, and set up camp.

Count me in amongst the people who stare at lumps of seaweed.

 

Who’s there? The whole lump is ~12 cm.

How many animals do you see here? Which ones appeal to you?

I have made a quick annotation of some of the biota here:

Note that these are just some of the critters present…! (photo: K. Kongshavn)

Let’s go closer on a small piece of algae:

Now, what do you see? (photo: K. Kongshavn)

For Luis, the first thing to catch the eye was (of course) the Hydrozoa

Hydrozoans (the christmas light looking strings), encrusting bryozoans (the flat, encrusting growth on on the algae – you might also know them as moss animals), and some white, spiralling polychaete tubes  (photo: K. Kongshavn)

Did you spot the sea hare (Aplysia punctata?) Look a bit above the middle of the photo of the tiny aquarium with the black background. Do you see a red-pink blob?

Hello, Aplysia punctata! (photo: K. Kongshavn)

There were also several other sea slugs that I have handed over to Cessa for inclusion in the sea slugs of Southern Norway project, here are a few:

Then there were the shelled gastropods:

The brittle star from the earlier image – this is a Ophiopholis aculeata, the crevice sea star (photo: K. Kongshavn)

In fact, they both are Ophiopholis aculeata (in Norwegian we call them “chameleon brittle stars” – they live up to the name!), one of the very common species around here. (photo: K. Kongshavn)

One of the colonial ascidian tunicates (and some of the ever present bryozoa just below it) (photo: K. Kongshavn)

Most of these animals will be barcoded, and will help build our reference library for species that occur in Norway. I also hope that they may have helped open your eyes to some of the more inconspicuous creatures that live just beneath the surface?

2019 will see the start of a new species taxonomy project where we will explore the invertebrate fauna of shallow-water rocky shores, so there will be many more posts like this to come!

-Katrine

Bryozoan barcoding

Haeckel Bryozoa.jpg
By Ernst Haeckel – Kunstformen der Natur (1904), plate 23: Bryozoa (see here, here and here), Public Domain, Link You can also find the whole, gorgeous book by Haeckel here, courtesy of the Biodiversity Heritage Library

I have spent the past week and a half getting acquainted with a rather odd – yet beautiful – group of animals, the Bryozoa, moss animals. These colony-forming, mostly marine, animals are small as individuals, but the colonies can grow quite large. Globally there are around 5000 extant species recorded, with a further 15 000 species in the fossil record. We have colleagues in Oslo who work on both the fossil and the current fauna to better understand micro- and macroevolution, you can read more about that here (og her, på norsk).

Sampling site of barcoded Bryozoans in the BOLD database

Sampling site of barcoded Bryozoans in the BOLD database

This is the first attempt at barcoding bryozoans through NorBOL, and it shows (map above); hopefully we will get more dots on the map for our region soon!

This may not be an easy group to get genetic barcodes from, though – I’ve been in communication with several of the (wonderfully helpful!) experts in the field, and the consensus seems to be that getting a barcode (from the region defined as THE barcode, the 5’ end of COI) will be difficult, and that we may anticipate “..a colourful array of contaminants, as well as nuclear mitochondrial pseudogenes”. Yay. Well, we won’t know until we try!

Together with colleagues from the Natural History Collections in Gothenburg we have assembled a plate of tissue samples from Swedish and Norwegian bryozoan that I will send to the CCDB facilities for sequencing next week. We have an impressive 58 different species (1-3 specimens of each) included on the plate, as well as a few specimens that are (not yet) identified to species.

n344_w1150

Bicellariella ciliata for barcoding

Bicellariella ciliata for barcoding

The colonies can be branching, encrusting, lacelike, lumpy…and at times pretty close to invisible! I’ve had to spend some time looking for good illustrations to know what to sample from… there are often multiple species in a jar, as well as other animals – hopefully I managed.

The specimens on the plate

The specimens on the plate

We’re treating this as a trial plate: is it possible to barcode museum material of bryozoans through the general pipeline, or will we need to get creative?

I’ll make a new post once the verdict is in – let’s hope for surprisingly high success rates!


Some further reading:

Lee et al 2011: DNA Barcode Examination of Bryozoa (Class: Gymnolaemata) in Korean SeawaterKorean J. Syst. Zool. Vol. 27, No. 2: 159-163, July 2011 ISSN 2233-7687
DOI 10.5635/KJSZ.2011.27.2.159

Wikipedia has a nice post on Bryozoa