Category Archives: Lower Heterobranchia and Pyramidellidae

Snail heaven in Sletvik!

From the 13th to 20th of October, we were on fieldwork again! This time the end destination was Sletvik field station. Sletvik field station belongs to the Norwegian University of Science and Technology in Trondheim (NTNU). The team of NorDigBryo (digitization of Norwegian Bryozoa) had organized a workshop there and team snail was invited to tag along for the opportunity to collect some snails around the area. So there the three of us traveled from Bergen up North; Jon and Katrine for the Bryozoan workshop and me for the Lower Heterobranchia and Pyramidellidae project.

The travel from Bergen to Trondheim takes more than 10 hours! For such a long travel we of course needed to take several breaks throughout the day. But with a bunch of biologists on the way it was very difficult to not sample during those stops whenever we had the opportunity (1).

two people on a pier in raingear, and a colourful nudibranch held in a hand

1. Sampling on our way, together with Jon & Katrine visiting several harbors. Photo: Cessa Rauch, UiB.

After a very long day, we finally arrived at our end destination; Sletvik field station. This would be our home for the coming week. The station has great facilities with different laboratories, a cantina with 3 meals a day being served by the kitchen staff and sleeping facilities. There is space for up to 40 students, so with just 10 of us we had a ton of space (2).

the field station, a large yellow wooden building

2. The Sletvik field station from the Norwegian University of Science and Technology in Trondheim (NTNU). Photo: Cessa Rauch, UiB.

The Sletvik field station is located on the small peninsula called Slettvik; surrounded by mostly water makes it an excellent location for marine related fieldwork. Despite the relatively small size of the peninsula, it has a surprising number of different habitats; there are seagrass meadows, sea bottoms covered in encrusting coralline algae and due to strong tidal currents, a very vibrant and diverse marine life (3).

a collage of six photos showing different marine shallow water habitats

3. Sampling from land…or in the water! From different marine habitats! Photos: Cessa Rauch, UiB.

Therefore, we used several days that week to collect fresh material from around the area: by using nets or hands either from land or while in the water snorkeling (4).

two people in black wet suits standing in the water, in a pretty sunset

4. Snorkeling for samples in strong tidal current right under the little bridge, with Jon and Cessa. Photo Katrine Kongshavn, UiB.

The strong tidal currents are what a lot of the Pyramidellid species absolutely love! Not the least because it attracts high diversity of their hosts that they parasite on (5). Places with lot of current have large influx of nutrients and are well oxygenated which often results in high diversity, such as the well-known Saltstraumen area in Nordland. Therefore, it was easy to collect them as the snails were so abundant.

a tiny snail, just a few millimeters, amongst other animals

5. Hard to spot the small snails, here Odostomia turrita (blue circle) crawling away from its host Serpullid worm (white with blue fringes). Photo: Cessa Rauch, UiB.

But it became clear quickly that a few species were quite dominant. The four most common Pyramidellid species in the shallow tidal currents around Sletvik were Odostomia turrita; Brachystomia scalaris; Spiralina spiralis and Parthenina intersincta (6). Although Pyramidellidae snails are often very difficult to identify, these fours exhibited very typical characteristics which made it somewhat easy to name them to species level (7).

4 small snails

6. 4 of the most common species found in Sletvik: Left up; Odostomia turrita, left down; Brachystomia scalaris, right up; Spiralina spiralis, right down; Parthenina intersincta. Photo: Cessa Rauch, UiB

hand drawn figure showing the key characters to tell four similar species of small snails a part

7. Doodles of the most recognizable characters of different common species in Sletvik. Photo: Cessa Rauch, UiB.

However, we still will extract DNA from these animals to confirm species, as it still can happen that we are having snails that are very similar and might have been misidentified in the field. That is why it is important to fix the collected snails in ethanol, so the tissue and DNA in it stays preserved. All collected material will then go back to the University Museum of Bergen to be further used for microscopy, morphological analysis, DNA extractions and eventually become part of the collection of the museum.

After one week, with hours of sorting through collected material, we managed to collect and identify 15 different species; the most so far of any fieldwork so we can say that Sletvik is truly a snail heaven!

– Cessa

Viberodden marine biodiversity workshop

After the Summer break the hunt for small snails continues. This time the museum staff got invited by Vivian Husa from the Institute of Marine Research and Erling Svensen underwater photographer and author of Marine fish & invertebrates of Northern Europe to come and help with marine biodiversity assessment in Egersund.

Egersund is a small village at the southwest side of Norway and strangely has almost no difference in height between low and high tide. The tidal range is close to zero due to the presence of a tidal node, which there are several off around the globe. This all makes Egersund a very interesting place to hunt for snails (and other marine organisms)!

Our stay was at a very special place; a lighthouse, called Viberodden (1).

Vibberodden, Eigersund

(1) Vibberodden fyr from the Eigersund Arkivinstitusjon.

The Viberodden lighthouse is build on a small island 15 minutes’ drive with boat from Egersund city center. Today it is run by the so-called Viberodden friends’ foundation (Viberodden venner). They rent out the lighthouse to be able to cover the costs for maintenance and cultural activities.  The lighthouse was originally built to secure entrance to Egersund in 1855 and has been upgraded several times since. The lighthouse was manned until 1977, when it became fully automatic. In 2007 the Viberdden friends’ foundation rehabilitated the entire lighthouse, and they are still in charge of upkeeping today. We therefore also want to thank the foundation for the hospitality and the possibility for us to use the lighthouse as a research station for the week!

Once we arrived in Egersund, Erling would be waiting for us with his boat to drive us and a whole lot of equipment to the lighthouse (2).

Two people in a boat, looking forward over the water

(2) Erling Svensen (left) and Vivian Husa (right) organizers of the workshop on their way to the lighthouse. Photo Cessa Rauch, UiB.

During the week, we grew in number of participants and eventually the team existed of a mix of researchers from different institutions and companies with a variety of backgrounds. We had with us macroalgae, Bryozoan (moss animal), Ascidian (sea squirts), sea slug specialists and more (3).

tree men looking at a computer screen (they are studying photos taken that day)

(3) From left to right Bjørn Gulliksen professor emeritus from the Norwegian Arctic University, Erling Svensen and Bernard Picton curator of marine invertebrates, National Museums Northern Ireland, together in discussion. Photo Katrine Kongshavn, UiB.

From the museum we were also representatives of several different Norwegian taxonomy initiatives (artsprosjektet).  There was “Digitization of Norwegian Bryozoans” (NorDigBryo), “Marine Amphipods: Diversity, Species Complex, and Molecular Studies” (MADAM) and of course “Lower Heterobranchia and Pyramidellidae of Norway”.

During the week we would collect several different habitats, from exposed kelp forests to blue mussel beds in the Egersund harbour. The lighthouse itself was built far out and therefore very exposed hence we were dependent on the weather for us to go out and collect. Luckily, we had a few good weather days that allowed us to snorkel while collecting samples (4).

Two women in wetsuits, ready for the water

(4) Snorkelling with Mette Eilertsen from Rådgivende Biologer AS. Photo Cessa Rauch, UiB.

To get our specimens we collected different habitats by using jars, nets and buckets and amazingly all our samples turned out to be rich in species (5 & 6).

A person scrubbing mussels with a brush to dislodge animals living on the outside of it

(7) Brushing mussels to get the little snails. Photo Katrine Kongshavn, UiB.

 

 

While searching for snails there was a clear preference of certain species to inhabit either exposed or sheltered environments, kelp or sandy bottoms. In one case we had to brush a lot of blue mussels to get to the Pyramidellidae (Odostomia turrita) that parasite on them and on the worms (Serpulidae) that grow their houses on the outside of the blue mussel shells (7).

But we also found certain species that crawled around in the mud (8) and others that were on the kelp (9).

A small snail (3 mm) on a black background

(8) Brachystomia sp. Photo Katrine Kongshavn, UiB.

A small snail climbing on algae

(9) Odostomia turrita. Photo Cessa Rauch, UiB.

The days were long, and we had to do a lot of sorting of the many materials that were collected (10).

(10) Sorting samples Cessa (left) and Jon Kongsrud (right). Photo Katrine Kongshavn, UiB.

But it was very much worth it as we found a range of interesting species that we did not collect in earlier fieldtrips from this year (11 & 12).

All these new findings and collection building would not have been possible without the help of the organizers, participants and the possibility for us to stay at the Viberodden lighthouse and therefore we would like to thank Erling Svensen, Vivian Husa, Bernard Picton, Mette Eilertsen, Bjørn Gulliksen and Viberodden friends’ foundation! Thank you for a great and productive week!

-Cessa

Minute snails in Northern Norway

From 21 till 28th of May, researchers, technicians, and students of the University Museum’s marine section, travelled up North to Torsvåg, close to Tromsø, for joint fieldwork. The participants represented several Artsdatabanken projects that cover marine fungi, hydrozoans, polychaetes, parasites of jellyfish, comb jellies and chaetognaths, bryozoans, marine amphipods and finally the Lower Heterobranchia and Pyramidellidae gastropods. In this blog you can read about the general experience of the fieldwork and more details about the different projects. And here you can read about the fieldwork through the eyes of two master students who joined. And if you want to read more adventures and see more pictures check out this blog post! It was a large group of young and more experienced scientists which created the perfect opportunity for a lot of knowledge transfer.

This was also the first “big” fieldwork trip for the Lower Heterobranchia and Pyramidellidae project, after several sampling events in the Bergen area, which you can read here. Both Lower Heterobranchia and Pyramidellidae include small snails, just a few millimetres in length that are hard to identify (they can resemble other small species of gastropods). The diversity of these tiny sea snails is poorly understood in Norway, and thus, during this project these sea snails will be studied by combining DNA barcoding and shell characters. Sampling will be based on the use of dredges, grabs, and snorkelling.

Pyramidellidae are regarded as ectoparasites and are often found living on other molluscs or other marine invertebrates, but also free in soft sediments. The Lower Heterobranchia are often found on algae, for example on the stipes and in between the holdfasts of large kelp. Because of the small size of these snails the best way to collect them is by sampling the substrate they live on. So, this is what we did, we went snorkelling several times in the cold waters of Northern Norway, but thanks to good neoprene layers we were able to keep warm and simultaneously looking like seals!

Underwater shot of a person snorkelling, collecting algae just below the surface

Collecting the right substrates for the Lower Heterobranchs and Pyramidellidae while snorkeling. Photo by Eva Charlotte Samson, UiB

Little lab set-up in the kitchen to sort through the samples.

Little lab set-up in the kitchen to sort through the samples. Photo by Cessa Rauch, UiB.

 

It was challenging to find our snails; there was plenty of kelp and high diversity of many other taxonomic groups, but the conditions were not exactly right especially for the pyramidellids that seem to prefer areas with strong currents.

So, even though we sampled many different habitats, we often ended up not finding our snails when back in the lab sorting under the microscope.

 

 

 

A tiny snail lookin up at the camera

Species of Pyramidellidae; Odostomia turrita. Photo by Cessa Rauch, UiB.

 

Yet, after collecting a ton of material and spending many hours sorting, we finally found one pyramidellid!

In this case Odostomia turrita.

Odostomia are neat little pyramidellids that have glands with distinct colours, which makes somewhat easier the identification of species.

Drawings showing the colour patterns of snails

The different colour patterns distinguish different Odostomia species. Source from Høisæter 2014. (Høisæter, T. (2014). The Pyramidellidae (Gastropoda, Heterobranchia) of Norway and adjacent waters. A taxonomic review).

Although minute, the lower heterobranch were “easier” to find… They seem to be less picky with the environment, and on kelp and sand we managed to sample a few different species, amongst others two very similar ones: Ammonicera rota and Omalogyra atomus. In addition, we found in the sand and gravel small snails of the genus Rissoella – R. globularis.

Field season has just started, this was a good beginning for a busy Summer with many more blogs to come!

-Cessa & Manuel

Fieldwork at Torsvåg Lighthouse (Troms)

map of Norway with a marker indicating Torsvåg, a bit north of Tromsø

Torsvåg lighthouse. Graphic from norgeskart.no)

 

During the last week of May, eleven of us in “the marine group” went on fieldwork together, up far north.

Based in Torsvåg (see map), we’ve collected material for several species mapping projects supported by the Norwegian Taxonomy Initiative (Artsprosjektet).

 

group photo of th eparticipants - 11 scientists in outdoor clothing standing in front of the ocean

The UMB participants. From back left: Joan, Jon, Tom, Nataliya, Katrine, Cessa, Marta (guest of NOAH). Front from left: Lea, Luis, Eva and Praveen

The fieldwork originated from the collaboration between the projects “Norwegian Marine Fungi” (Teppo Rämä, UiT), “NOAH – Norwegian Arctic Hydrozoans” (Joan J. Soto-Àngel, UMB), and “Polychaetes in the Arctic” (ManDAriN) (Nataliya Budaeva, UMB).

Additionally, the projects “Lower Heterobranchia and Pyramidellidae of Norway,” “Parasites on Jellyfish, Comb Jellies, and Chetognaths” (ParaZoo), “Digitization of Norwegian Bryozoans” (NorDigBryo), and “Marine Amphipods: Diversity, Species Complex, and Molecular Studies” (MADAM) were represented (many of us participate in multiple projects), covering a wide range of organisms.

The light house, and some signs that you may have been invaded by marine biologists (photo: K. Kongshavn)

Weather-wise, the Bergen team had some doubts as we packed our car in nearly 30-degree heat while snow was falling up North. However, it turned out that good weather awaited us! By the time we settled into our accommodations, the spring-winter was over, the sun came out – and didn’t set! An advantage of being in the land of the midnight sun, for sure.

four images: two from plane window going from summer (bergen) to winter (tromsø), two screen shot of the weather reporting in media

From summer to winter – or? (photos: K. Kongshavn, screengrabs from yr.no and nrk.no)

The scenery was breathtaking!
Upon arriving at Torsvåg Lighthouse, Teppo treated us to pancakes and a strategy meeting. Before the evening was over, we had our first samples in the lab, collected at low tide near the lighthouse.

scenic shot of tidepool and snow covered mountains

Our first colleting of the trip was done in these tide pools. Photo: K. Kongshavn

From there, it was a flurry of activity: collecting from tide pools, docks, marinas, kelp forests, and boats. We had one day of suboptimal weather with too much wind for extensive outdoor work, but by then we certainly had enough material to keep us busy indoors.

a collage showing different gears and methods used for collecting samples

Sampling methods: Snorkelling, from boat, hand pick in the intertidal, using a (small!) grab, sieving and collecting from piers and marinas. Photos: K. Kongshavn

In total, there were 14 of us, spread across the lighthouse and two apartments.
The lab space was set up for fungi in the lighthouse, while the two apartments were home to “team cnidarians & ctenophores” and “team benthos” (polychaetes, snails, bryozoans, and amphipods). Sorting, identification, photography, and documentation took place, with samples migrating between labs as we discovered interesting finds.

a collage of different animals; a bristle worm, a tiny snail, an amphipod and a bryozoan colony

Some of our collected animals, a screenshot from the dealing of samples via WhatsApp, and a sample being sorted. Fotos: K. Kongshavn

We also had a nice visit from the Norwegian Biodiversity Information Centre (Artsdatabanken), who joined us for fieldwork and lab activities. Stay tuned for a news article—we’ll share the link when it’s available.

A big thank you to everyone for their great attitudes, willingness to share knowledge, samples, and boat time, as well as for all the delicious food made and fun memories created!

Keep an eye out for posts from the different projects in the near future!

–Katrine (on behalf of the travelers)

Project kick-off; Lower Heterobranchia and Pyramidellidae of Norway

Since September 2023, a new taxonomic project started at the University Museum studying the biodiversity of sea snails (with shell) and sea slugs (without (visible) shell) in Norwegian marine habitats. This project is in a way a follow up from the “Sea slugs of Southern Norway” project between 2018–2020, but this time with a focus on poorly studied habitats including seagrass meadows, maerl coralline algae beds and landlocked fjords (“polls”). The target groups of this project are tiny sea snails of just a few millimeters, which are part of a group called “Lower” Heterobranchia and Pyramidellidae (Image 1) that together with the former ophistobranchs (e.g. the sea lugs), and pulmonates (slugs and land snails) form the Heterobranchia, one of the main evolutionary lineages of the Gastropoda.

Snail shells on a black bacground

Image 1: Example of shells of a “Lower Heterobranchia” (A) and a Pyramidellidae species (B). Picture CRauch & MMalaquias.

The diversity of these tiny sea snails is poorly understood in Norway. Currently, nine families are known in the country: Acteonidae, Cimidae, Mathilidae, Murchiconellidae, Omalogyridae, Rissoellidae, Tjaernoeiidae, Xylodisculidae and Pyramidellidae (Høisæter 2014), but their small size and similar shells make it challenging to identify the species. During this project these sea snails will be studied in Norway for the first-time combining DNA barcoding and morphology. Sampling will be based on the use of dredges, grabs, and snorkeling by collecting the substrate they live on.

Pyramidellidae are ectoparasites and are often found living on other molluscs or other marine invertebrates, but also on soft sediments. The University Museum of Bergen has already a good collection of “Lower” Heterobranchia and Pyramidellidae from Norway, but a large part of this material is not suitable for DNA extraction due to the original fixation methods based on the use of formaldehyde. Therefore, after the first months of cataloguing the “old” collection and setting up protocols for DNA extraction, we finally set out in February to collect fresh material. We visited two different sites around Bergen to try out collecting specimens by snorkeling and test the efficiency of a recently acquired portable hand-dredge (Image 2).

A small metal dredge, rope and a buoy on a dock

Image 2: The hand dredge which was used for collecting specimens. Picture MMalaquias.

Our first fieldwork day took place at the Marine Biological Station of UiB at Espegrend. The field station is a convenient place as it features a fully equipped laboratory (Image 3), and just in front of the station there is a seagrass meadow dominated of Zostera marina. This eelgrass is often found in shallow and sheltered coastal areas between 0.5 m to 10 m depth on flat sandy bottoms. Seagrass meadows are diverse and productive and can harbor hundreds of associated species from many different groups of animals. One team member swam with the dredge over the seagrass meadow and dropped it about 30 m from the shoreline, while the other team member pulled it back by hand. In addition, substrates like algae, blue mussels, and ascidians were hand-picked by snorkeling.

A scientist in a lab, looking in a stereo microscope

Image 3: First fieldwork for the new project, with project leader Manuel Malaquias behind the stereomicroscope searching for sea snails and slugs at Espegrend Marine Biological Station. Picture CRauch.

These efforts yielded the first specimens, namely the “Lower” Heterobranch species Ammonicera rota (Image 4) and the sea slug Elysia viridis (Image 5).

During our second sampling event, because of bad weather conditions with strong southern winds, we decided to visit a sheltered popular swimming area (although at this time of the year not so popular!) located north of Bergen called Helleneset (Image 6). Helleneset is a rocky shore area rich in algae and kelp with sand flats in between.

Two people on a pier in wet weather

Image 6: Second fieldwork trip, at the popular swimming area called Helleneset, on a wet and cold February day deserted. In the picture Manuel Malaquias and Cessa Rauch. Picture CRauch & MMalaquias 

This time the algae and kelp mats collected by snorkeling in ziplock plastic bags did not yield much, but a close examination of the sand (with help of stereomicroscopes) collected again with the hand-dredge revealed the presence of many little gastropod snails. One of these little snails was our first Pyramidellidae; the identity is not yet completely confirmed, but it looks like Eulimella ventricosa (Image 7).

a small shelled gastropod on a black bacground

Image 7: A Pyramidellidae found from Helleneset, a possible Eulimella ventricosa. Picture MMalaquias.

These two first samplings days were a good test for how we best can collect these snails which are small and in general a little abundant. The dredge is easier to operate with two people working together; one to deploy it and the other to pull it back to shore. Sandy samples yield better results if left to rest for several hours and even days in trays or buckets because anoxic conditions begin to form, forcing the animals to crawl out of the sediment.

This is just the beginning, if you want to be part of the journey of the diversity of “Lower” Heterobranchia and Pyramidellidae in Norway, we will be regularly updating stories and findings on this channel, and our social media accounts @SeaslugsofNorway (both Instagram and Facebook), see you there!

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References

Høisæter, T. 2014. The Pyramidellidae (Gastropoda, Heterobranchia) of Norway and adjacent waters. A taxonomic review. Fauna Norvegica, 34: 7-78.